Peanut (Arachis hypogaea L.) lines containing the nucleocapsid protein (N) gene of tomato spotted wilt virus (TSWV) were generated via microprojectile bombardment of somatic embryos from the cultivar Okrun, a runner-type peanut primarily grown in the southwestern U.S. The gene for hygromycin resistance, hph, was included on the plasmid containing the N gene as a selectable marker. PCR and ELISA results indicated that eight of 10 primary transformant lines were positive for incorporation and expression of the TSWV N transgene. PCR and ELISA analyses of T1 progeny indicated that two of the eight transgenic lines demonstrated a 3:1 segregation ratio, indicative of incorporation of a single copy of the TSWV N transgene. Transgene expression levels remained constant through the T1 generation.
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Keywords: transformation, virus, peanut, resistance, TSWV
How to Cite:
Chenault, K. & Payton, M., (2003) “Genetic Transformation of a Runner-Type Peanut with the Nucleocapsid Gene of Tomato Spotted Wilt Virus”, Peanut Science 30(2), p.112-115. doi: https://doi.org/10.3146/pnut.30.2.0010