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Genetic Transformation of a Runner-Type Peanut with the Nucleocapsid Gene of Tomato Spotted Wilt Virus

Authors: K. D. Chenault , M. E. Payton

Abstract

Peanut (Arachis hypogaea L.) lines containing the nucleocapsid protein (N) gene of tomato spotted wilt virus (TSWV) were generated via microprojectile bombardment of somatic embryos from the cultivar Okrun, a runner-type peanut primarily grown in the southwestern U.S. The gene for hygromycin resistance, hph, was included on the plasmid containing the N gene as a selectable marker. PCR and ELISA results indicated that eight of 10 primary transformant lines were positive for incorporation and expression of the TSWV N transgene. PCR and ELISA analyses of T₁ progeny indicated that two of the eight transgenic lines demonstrated a 3:1 segregation ratio, indicative of incorporation of a single copy of the TSWV N transgene. Transgene expression levels remained constant through the T₁ generation.

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Keywords: transformation, virus, peanut, resistance, TSWV

How to Cite: Chenault, K. & Payton, M. (2003) “Genetic Transformation of a Runner-Type Peanut with the Nucleocapsid Gene of Tomato Spotted Wilt Virus”, Peanut Science. 30(2). doi: https://doi.org/10.3146/pnut.30.2.0010