1Research supported by the New Mexico Agricultural Experiment Station, New Mexico State Univ., Las Cruces, NM.
This study evaluated plant development via direct organogenesis from in vitro-cultured young seedling tissues of cultivated peanut, especially the valencia-type peanut. Complete plants were regenerated from in vitro-cultured petiolule-with-blade-attached explants, leaflet segments, and epicotyl andpetiole sections. Multiple shoots arose on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BA) (525 mgL) plus 1-naphthaleneacetic acid (NAA) (0.53 mgL). After 30 d culture on 25 mgL BA + 1 mgL NAA, 1.6 buds or shootsexplant were regenerated from the petiolule-with-blade-attached explants. Comparable numbers of shoots were obtained from epicotyl sections of the first node region of the seedling after 60 d culture using 10 mgL BA + 1 mgL NAA. Leaflet segments and petiole sections were less responsive for shoot formation. Excised shoots developed roots in vitro upon transfer for 15 d to MS medium supplemented with NAA at 1 mgL. Plantlets were transferred to soil and grown in a greenhouse to maturity. A wide range of cultivated peanut genotypes was evaluated for organogenic responsiveness, using the petiolule-with-blade-attached explant source. Only valencia-type cultivars, or a hybrid derivative with a Valencia background, were responsive with this regeneration system.
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Keywords: peanut, seedling tissues, in vitro, organogenesis, regeneration
How to Cite:
Cheng, M. & Hsi, D. & Phillips, G., (1992) “In Vitro Regeneration of Valencia-type Peanut (Arachis hypogaea L.) from Cultured Petiolules, Epicotyl Sections and Other Seedling Explants¹”, Peanut Science 19(2), p.82-87. doi: https://doi.org/10.3146/i0095-3679-19-2-5