1Journal Article No. 4442, Oklahoma Agricultural Experiment Station, Oklahoma State University, Stillwater, OK.
An efficient procedure was developed for purification of peanut mottle virus (PMV) from pea (Pisum sativum cv. Little Marvel) that yielded 10-19 mg virus/kg infected tissue. Virus was extracted from frozen infected tissue in 0.01 M potassium phosphate buffer, pH 8.0, with 0.001 M dithioerythritol, followed by clarification with chloroform (15%, v/v) and precipitation by KCl and polyethylene glycol. Virus was resuspended in 0.01 M borate-phosphate buffer, pH 8.3, with 0.2 M urea prior to density gradient centrifugation. Purified virus sedimented as a single component with a sedimentation coefficient of 149 S. The molecular weight of the single coat protein was estimated as 36,100 daltons in 12% polyacrylamide gels. The single nucleic acid isolated from PMV on sucrose gradients was degraded by RNase, but not DNase. The molecular weight of the RNA was estimated as 3.1 × 106 daltons on nondenaturing and denaturing sucrose gradients.
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Keywords: Arachis chacoense, Groundnut, PMV, potyvirus
How to Cite:
Sherwood, J., (1984) “An Efficient Procedure for Purification of an Isolate of Peanut Mottle Virus from Wild Peanut and Determination of Molecular Weights of the Viral Components¹”, Peanut Science 11(1), p.40-42. doi: https://doi.org/10.3146/i0095-3679-11-1-12