Immature leaflets 3 to 5 mm in length from Arachis villosulicarpa Hoehne seedlings were cultured in vitro on a medium consisting of the major and minor salts of Murashige and Skoog (MS), Gamborg's B5 vitamins, 20 gL sucrose, 0.8 Difco agar, 4.44 M N-6 benzyladenine (BA) and 5.37 M napthaleneacetic acid (NAA). Hard callus with many shoot primordia formed on all leaflets within 4 to 6 weeks. Calli with primordia were further cultured for plant formation on 150 other media (MS major salts at 12 or 14 strength, full strength minor salts and vitamins, sucrose at 5 or 10 gL, BA and NAA at 0 to 8 M). Reduced concentrations of major salts with a high ratio of BA to NAA enhanced the formation of shoot primordia. Roots differentiated on media with 14 strength major salts, no BA, and 6 to 8 M NAA and 5 gL sucrose. Shoots differentiated from the branch points of roots after 2 to 4 months of culture. Plants were obtained via shoot primordia from callus and from the adventitious shoots from roots.
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Keywords: Groundnut, Morphogenesis, organogenesis, SEM, Tissue Culture
How to Cite:
Pittman, R. & Johnson, B. & Banks, D.,
(1984) “In Vitro Differentiation of a Wild Peanut, Arachis villosulicarpa Hoehne¹,²”,
Peanut Science 11(1),